CovidV1, Main, Exploration, bibRecord, 000E18

Effect of Recombinant Lactobacillus Expressing Canine GM-CSF on Immune Function in Dogs

Identifieur interne : 000E18 ( Main/Exploration ); précédent : 000E17; suivant : 000E19

Effect of Recombinant Lactobacillus Expressing Canine GM-CSF on Immune Function in Dogs

Auteurs : CHUNG JIN YOUNG [Corée du Sud] ; EUI JAE SUNG [Corée du Sud] ; CHUN GYU CHO [Corée du Sud] ; KYOUNG WON SEO [Corée du Sud] ; Jong-Soo Lee [États-Unis] ; DONG HA BHANG [Corée du Sud] ; HEE WOO LEE [Corée du Sud] ; CHEOL YONG HWANG [Corée du Sud] ; WAN KYU LEE [Corée du Sud] ; HWA YOUNG YOUN [Corée du Sud] ; CHUL JOONG KIM [Corée du Sud]

Source :

Journal of microbiology and biotechnology [ 1017-7825 ] ; 2009.

RBID : Pascal:10-0017099

Descripteurs français

Pascal (Inist)
- Lactobacillus, Facteur stimulant colonie granulocyte macrophage, Chien, In vitro, In vivo.

English descriptors

KwdEn :
- Dog, Granulocyte macrophage colony stimulating factor, In vitro, In vivo, Lactobacillus.

Abstract

Many Lactobacillus strains have been promoted as good probiotics for the prevention and treatment of diseases. We engineered recombinant Lactobacillus casei, producing biologically active canine granulocyte macrophage colony stimulating factor (cGM-CSF), and investigated its possibility as a good probiotic agent for dogs. Expression of the cGM-CSF protein in the recombinant Lactobacillus was confirmed by SDS-PAGE and Western blotting methods. For the in vivo study, 18 Beagle puppies of 7 weeks of age were divided into three groups; the control group was fed only on a regular diet and the two treatment groups were fed on a diet supplemented with either 1×10⁹ colony forming units (CFU)/day of L. casei or L. casei expressing cGM-CSF protein for 7 weeks. Body weight was measured, and fecal and blood samples were collected from the dogs during the experiment for the measurement of hematology, fecal immunoglobulin (Ig)A and IgG, circulating IgA and IgG, and canine corona virus (CCV)-specific IgG There were no differences in body weights among the groups, but monocyte counts in hematology and serum IgA were higher in the group receiving L. casei expressing cGM-CSF than in the other two groups. After the administration of CCV vaccine, CCV-specific IgG in serum increased more in the group supplemented with L. casei expressing cGM-CSF than the other two groups. This study shows that a dietary L. casei expressing cGM-CSF enhances specific immune functions at both the mucosal and systemic levels in puppies.

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Effect of Recombinant Lactobacillus Expressing Canine GM-CSF on Immune Function in Dogs</title>
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<front><div type="abstract" xml:lang="en">Many Lactobacillus strains have been promoted as good probiotics for the prevention and treatment of diseases. We engineered recombinant Lactobacillus casei, producing biologically active canine granulocyte macrophage colony stimulating factor (cGM-CSF), and investigated its possibility as a good probiotic agent for dogs. Expression of the cGM-CSF protein in the recombinant Lactobacillus was confirmed by SDS-PAGE and Western blotting methods. For the in vivo study, 18 Beagle puppies of 7 weeks of age were divided into three groups; the control group was fed only on a regular diet and the two treatment groups were fed on a diet supplemented with either 1×10<sup>9</sup>
 colony forming units (CFU)/day of L. casei or L. casei expressing cGM-CSF protein for 7 weeks. Body weight was measured, and fecal and blood samples were collected from the dogs during the experiment for the measurement of hematology, fecal immunoglobulin (Ig)A and IgG, circulating IgA and IgG, and canine corona virus (CCV)-specific IgG There were no differences in body weights among the groups, but monocyte counts in hematology and serum IgA were higher in the group receiving L. casei expressing cGM-CSF than in the other two groups. After the administration of CCV vaccine, CCV-specific IgG in serum increased more in the group supplemented with L. casei expressing cGM-CSF than the other two groups. This study shows that a dietary L. casei expressing cGM-CSF enhances specific immune functions at both the mucosal and systemic levels in puppies.</div>
</front>
</TEI>
<affiliations><list><country><li>Corée du Sud</li>
<li>États-Unis</li>
</country>
<region><li>Californie</li>
<li>Région capitale de Séoul</li>
</region>
<settlement><li>Los Angeles</li>
<li>Séoul</li>
</settlement>
<orgName><li>Université de Californie du Sud</li>
<li>Université nationale de Séoul</li>
</orgName>
</list>
<tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Chung Jin Young" sort="Chung Jin Young" uniqKey="Chung Jin Young" last="Chung Jin Young">CHUNG JIN YOUNG</name>
</region>
<name sortKey="Cheol Yong Hwang" sort="Cheol Yong Hwang" uniqKey="Cheol Yong Hwang" last="Cheol Yong Hwang">CHEOL YONG HWANG</name>
<name sortKey="Chul Joong Kim" sort="Chul Joong Kim" uniqKey="Chul Joong Kim" last="Chul Joong Kim">CHUL JOONG KIM</name>
<name sortKey="Chun Gyu Cho" sort="Chun Gyu Cho" uniqKey="Chun Gyu Cho" last="Chun Gyu Cho">CHUN GYU CHO</name>
<name sortKey="Dong Ha Bhang" sort="Dong Ha Bhang" uniqKey="Dong Ha Bhang" last="Dong Ha Bhang">DONG HA BHANG</name>
<name sortKey="Eui Jae Sung" sort="Eui Jae Sung" uniqKey="Eui Jae Sung" last="Eui Jae Sung">EUI JAE SUNG</name>
<name sortKey="Hee Woo Lee" sort="Hee Woo Lee" uniqKey="Hee Woo Lee" last="Hee Woo Lee">HEE WOO LEE</name>
<name sortKey="Hwa Young Youn" sort="Hwa Young Youn" uniqKey="Hwa Young Youn" last="Hwa Young Youn">HWA YOUNG YOUN</name>
<name sortKey="Kyoung Won Seo" sort="Kyoung Won Seo" uniqKey="Kyoung Won Seo" last="Kyoung Won Seo">KYOUNG WON SEO</name>
<name sortKey="Wan Kyu Lee" sort="Wan Kyu Lee" uniqKey="Wan Kyu Lee" last="Wan Kyu Lee">WAN KYU LEE</name>
</country>
<country name="États-Unis"><region name="Californie"><name sortKey="Lee, Jong Soo" sort="Lee, Jong Soo" uniqKey="Lee J" first="Jong-Soo" last="Lee">Jong-Soo Lee</name>
</region>
</country>
</tree>
</affiliations>
</record>

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Effect of Recombinant Lactobacillus Expressing Canine GM-CSF on Immune Function in Dogs

Effect of Recombinant Lactobacillus Expressing Canine GM-CSF on Immune Function in Dogs

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